Aaron Davis was a burglar and career criminal. His brother used to be his partner in crime. Whereas his brother reformed when he became a father, Aaron continued his criminal activities as the Prowler and became estranged from his brother. While stealing technology from Osborn Industries under orders from Roxxon, a spider genetically enhanced with the Oz formula crawled into his bag and later bit Miles Morales, granting his nephew powers and turning him into the new Spider-Man.

While Davis was in Mexico settling accounts with the Scorpion, he was imprisoned. He escaped and went to the Tinkerer's hideout to blame him for the equipment he made. When both talked about Peter Parker's origin as Spider-Man, Aaron realized the spider that bit Miles in his house was from Osborn Industries, and that Miles was the new Spider-Man. To keep it a secret, Davis shot Tinkerer and went to visit Miles.

Using Miles' secret, he blackmailed his own nephew into helping him defeat Scorpion. After Scorpion was defeated by the joined forces of Spider-Man and Prowler, he tried to force Miles to continue working for him. Miles made an ultimatum where his uncle was to stay away from his family and to leave New York City forever, Davis decided to use his vibro-shock gauntlets to eliminate Miles. At the climax of the battle, one of Aaron's damaged vibro-shock gauntlets exploded. The blast left Aaron severely injured. Before losing consciousness, Aaron cryptically told Miles that he was very much like him as Miles could only look on in horror at his uncle's state. Davis was reported to have died from his injuries the following day